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[Ligand of platelet and T cell activation antigen 1 (PTA1) expressed on Colo205 cell surface]
1Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Objective:
To confirm the existence of PTA1 ligand (PTA1L).
Methods:
PTA1/Ig fusion protein was prepared by gene recombination and expression in COS-7 cells by DEAE-dextran transfection. The fusion protein was used as a probe in the investigation of PTA1L by immunohistochemical staining. Existence of PTA1L was further identified by adhesion experiment.
Results:
Colo205 cells could be stained by PTA1/Ig fusion protein specifically but not by hIg, and the adhesion of activated Jurkat cells with Colo205 could be blocked by PTA1/Ig and PTA1 mAb.
Conclusion:
PTA1 ligand is present on the surface of Colo205 cells. These results provide a solid basis for further investigation of the role of PTA1-PTA1L interaction in the pathogenesis of neoplasm.