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[Bcl-2 antisense oligodeoxyribonucleotide increases apoptosis of lung carcinoma cells induced by cisplatin]
Objective:
To study the effect of antisense oligodeoxyribonucleotide (ODN) to apoptotic suppress gene bcl-2 on apoptosis of lung carcinoma cells induced by cisplatin.
Methods:
The lung carcinoma cells expressing bcl-2 were chosen to participate in this experiment. Cultured cells were divided into 7 groups: ODN, nonsense, ODN + cisplatin, nonsense + cisplatin, cisplatin, lipofectin and control. Bcl-2 antisense or nonsense mixed with lipofectin was added into above corresponding cultured cells. After cultured for 6 hours, cisplatin was added into corresponding groups. The cells were cultured again for 16 hours. And then, the cells were smeared on slides. Apoptotic cells were labeled with TdT-mediated dUTP nick end labeling (TUNEL) method on cell smears. Apoptotic index (AI) was counted to show the percentage of apoptotic cancer cells. The immunocytochemistry was used to detect the expression of bcl-2 in carcinoma cells.
Results:
The bcl-2 expression of cancer cell in ODN group was significantly decreased compared to the control and nonsense groups. The AI of ODN + cisplatin group was 16.4 +/- 1.7, cisplatin group 4.1 +/- 0.8, antisense group 5.9 +/- 0.2, nonsense group 3.3 +/- 0.7, nonsense + cisplatin 7.6 +/- 1.1, lipofectin 5.1 +/- 0.9, control group 3.6 +/- 0.6. The AI of antisense + cisplatin group was significantly higher than that of other groups.
Conclusion:
Antisense oligodeoxyribonucleotide to bcl-2 can inhibit significantly the expression of bcl-2 of lung cancer cells and increase apoptosis of cancer cells induced by cisplatin.
Insights
Antisense oligodeoxyribonucleotide targeting the bcl-2 gene significantly reduces bcl-2 expression in lung cancer cells. This inhibition enhances cisplatin-induced apoptosis, offering a potential therapeutic strategy for lung carcinoma.
Area of Science:
- Molecular Biology
- Cancer Research
- Apoptosis Studies
Context:
- Lung carcinoma is a leading cause of cancer-related mortality.
- The bcl-2 gene plays a crucial role in inhibiting apoptosis.
- Targeting anti-apoptotic genes is a promising strategy in cancer therapy.
Purpose:
- To investigate the effect of antisense oligodeoxyribonucleotide (ODN) against the bcl-2 gene on lung carcinoma cell apoptosis.
- To evaluate the combined effect of bcl-2 antisense ODN and cisplatin on cancer cell death.
Summary:
- Lung carcinoma cells were treated with bcl-2 antisense ODN and/or cisplatin.
- The TdT-mediated dUTP nick end labeling (TUNEL) method was used to quantify apoptosis.
- Bcl-2 expression was assessed using immunocytochemistry.
Impact:
- Antisense ODN significantly inhibited bcl-2 expression in lung cancer cells.
- The combination of bcl-2 antisense ODN and cisplatin markedly increased the apoptotic index.
- This suggests a potential therapeutic approach for enhancing lung cancer treatment.