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[Drug susceptibility testing for M. tuberculosis with proportion method].

Y Liu1, S Wang, X Na

  • 1Beijing Tuberculosis & Thoracic Tumor Institute, Beijing 101149, China.

Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases
|January 10, 2002
PubMed
Summary

The proportion method for drug susceptibility testing (DST) of Mycobacterium tuberculosis (MTB) shows higher agreement for isoniazid and ethambutol compared to the absolute concentration method. Chinese anti-TB drugs are suitable for DST.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Pharmaceutical Sciences

Context:

  • Drug susceptibility testing (DST) is crucial for managing tuberculosis (TB).
  • The proportion method and absolute concentration method are standard techniques for MTB DST.
  • Evaluating the performance of different DST methods and drug sources is essential for accurate diagnosis.

Purpose:

  • To assess the feasibility and accuracy of the proportion method for Mycobacterium tuberculosis (MTB) drug susceptibility testing (DST).
  • To compare the proportion method with the absolute concentration method using both Chinese and Sigma-produced anti-TB drugs.
  • To investigate the influence of critical drug concentrations on DST method discrepancies.

Summary:

  • Drug susceptibility testing (DST) was performed on 360 MTB strains using proportion and absolute concentration methods.

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  • The proportion method demonstrated significantly higher resistance and accordance rates for isoniazid (INH) and ethambutol (EMB) compared to the absolute concentration method (P < 0.01).
  • No significant differences were observed for streptomycin (SM) and rifampicin (RFP). Accurances were 81.1% (INH), 92.5% (SM), 83.6% (EMB), and 98.6% (RFP). DST results were comparable between Chinese and Sigma-produced anti-TB drugs.

    • Impact:

    • The findings suggest that the proportion method is a feasible and reliable approach for DST of MTB, particularly for INH and EMB.
    • Critical drug concentrations are identified as the primary factor influencing discrepancies between the proportion and absolute concentration methods.
    • Chinese-manufactured anti-TB drugs are validated as suitable for DST, supporting their use in clinical settings and potentially reducing costs.