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Updated: Oct 3, 2026
Magnetic Resonance Imaging Assessment of Carcinogen-induced Murine Bladder Tumors
Published on: March 29, 2019
[Establishment of MRP-overexpression subline of bladder carcinoma and its MDR phenotype]
1Department of Urology, Xiehe Hospital, Tongji Medical University, Wuhan 430022, China.
Objective:
To investigate MRP expression and multidrug resistance(MDR) phenotype of a bladder carcinoma subline transfected with the full length MRP cDNA.
Methods:
After transfection, a stable MRP-overexpressed subline named EJ/MRP was established. Gene expression of MRP and mdr1 were detected by using RT-PCR and immunohistochemistry methods. Drug sensitivity testing of the EJ/MRP cells to 11 kinds of anti-cancer agents was examined.
Results:
Compared with EJ/Vect which was mock transfected, MRP mRNA level of EJ/MRP increased 14.3 fold and MRP expression was mainly located in cytosol and plasma membrane. The relative resistance (RR) to VP-16, vincristine increased more than 10 fold, and that to doxorubicin, hydroxycamptothecin, thiotepa, mitomycin and cyclophosphamide increased 3 to 10 fold.
Conclusion:
Bladder carcinoma with stably over-expressed MRP presents typical MDR phenotype but without mdr1/P-gp expression. It provides a good model and positive control for the study of MRP-mediated MDR.
