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Cationic antimicrobial protein of Mr 37 kDa: a multifunctional inflammatory protein

H A Pereira1

  • 1Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma 73190, USA. Anne-Pereira@ouhsc.edu

Chinese Medical Journal
|January 10, 2002
PubMed
Abstract

Insights

Cationic antimicrobial protein (CAP37) from neutrophils modulates endothelial cell function, increasing monocyte adhesion and migration. CAP37 is induced by inflammatory signals, highlighting its role in monocyte-endothelial interactions.

Area of Science:

  • Immunology
  • Cell Biology
  • Vascular Biology

Background:

  • Neutrophils release cationic antimicrobial protein of Mr 37 kDa (CAP37), an inflammatory mediator.
  • The function of CAP37 in endothelial cell biology is not fully understood.

Purpose of the Study:

  • To investigate the role of CAP37 in endothelial cell function.
  • To determine CAP37's effect on monocyte-endothelial cell interactions.

Main Methods:

  • Endothelial cells from human lung microvessels and rat aorta were used.
  • Protein kinase C activity, cell migration, and adhesion molecule expression (ICAM-1, PECAM-1) were measured.
  • RT-PCR was employed to detect CAP37 expression in endothelial cells.

Main Results:

  • CAP37 activated endothelial protein kinase C in a dose- and time-dependent manner.
  • CAP37 enhanced monocyte adhesion to the endothelium by upregulating ICAM-1 and PECAM-1.
  • CAP37 promoted endothelial cell migration and was induced by inflammatory cytokines and LPS.

Conclusions:

  • CAP37 plays a critical role in monocyte-endothelial cell interactions.
  • CAP37 facilitates monocyte transmigration into tissues.

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