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Relationship between tumor suppressor gene p53 and tumors of adipose tissue
1Department of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou 510089, China.
Objective:
To investigate the relationship between p53 gene and tumors of adipose tissue at the level of protein and gene.
Methods:
Immunohistochemical LSAB, PCR-SSCP and DNA sequencing were used in 82 cases.
Results:
p53 protein is expressed only in liposarcomas, in which the positive staining rate was 48.08% (25/52). In different subtypes of liposarcomas, the positive staining rate in well differentiated liposarcomas was 30.00% (9/30), which is much lower than that of the poorly differentiated liposarcomas (P < 0.005). Abnormality in the single-stranded DNA pattern was determined in 2 samples (pleomophic liposarcomas) by PCR-SSCP analysis. Missense mutations in exon 8 codon 268 of p53 gene (AAC-->ATC) were detected by DNA sequencing. Another heterozygotic cosense mutation may exist at exon 6 codon 221 of p53 gene (GAG-->GAA).
Conclusions:
The data suggest that the p53 protein has a relationship with development, differentiation and malignancy of liposarcoma. Detecting the level of p53 protein expression may be valuable in evaluating the level of differentiation and malignancy of liposarcoma. There appear point mutation on exon 8, 6 of p53 gene.