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Related Experiment Video

Updated: Jul 4, 2026

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

Published on: September 28, 2019

3D-computer based reconstructions of apoptotic nuclei.

J Peychl¹, J Husak, H Spring

¹Department of Medical Biology and Genetics, Charles University Faculty of Medicine in Hradec Kralove, Simkova 870,500 01 Hradec Kralove, Czech Republic. peychl@lfhk.cuni.cz

Frontiers in Bioscience : a Journal and Virtual Library

|January 10, 2002

Summary

This summary is machine-generated.

This study visualizes apoptotic cell nuclei in HL-60 cells treated with Etoposide. It reveals chromatin fragmentation and aggregation within the nuclear membrane, offering insights into programmed cell death.

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Area of Science:

Cell biology
Biophysics
Molecular oncology

Background:

Apoptosis is a crucial process in multicellular organisms.
Understanding nuclear morphological changes during apoptosis is vital for disease research.
Etoposide is a chemotherapeutic agent that induces DNA damage and apoptosis.

Purpose of the Study:

To create a 3D model of apoptotic nuclei in HL-60 cells.
To visualize the morphological changes of chromatin during Etoposide-induced apoptosis.
To analyze chromatin condensation and aggregation within the nuclear membrane.

Main Methods:

HL-60 cells were treated with 10 µg/ml Etoposide for 24 hours.
Confocal microscopy was used to obtain optical sections of the cells.
Freeware and shareware graphical programs were utilized for 3D model generation.
3D Studio Max was employed for animation of the 3D nuclear model.

Main Results:

A detailed 3D model of apoptotic HL-60 cell nuclei was successfully generated.
The model demonstrated characteristic chromatin fragmentation and condensation.
Chromatin was observed to aggregate towards the inner nuclear membrane.
Dynamic visualization highlighted the dynamic process of nuclear fragmentation.

Conclusions:

The 3D model provides a comprehensive view of nuclear morphological changes during Etoposide-induced apoptosis.
Chromatin aggregation at the nuclear periphery is a key feature of apoptosis in these cells.
This visualization technique aids in understanding the spatial dynamics of apoptotic processes.