Related Experiment Videos
Retinoic acid regulated insulin-like growth factor gene expression in cord blood lymphocytes
1Pediatrics Hospital, Shanghai Medical University, Shanghai 200032, China. yyang@shmu.edu.cn
Chinese Medical Journal
|January 10, 2002
Abstract
Objective:
To explore the immune promotion mechanism of retinoic acid (RA).
Methods:
Reverse Transcription-Polymerase chain reaction (RT-PCR) was used to detect the gene expression of insulin like growth factor I (IGF-I) and IGF receptors (IGF-IR and IGF-IIR) in vitro, and their regulation by RA in human cord blood lymphocyte (CBL).
Results:
Significant upregulation of IGF-I, IGF-IR and IGF-IIR mRNA was found at 6-24 hours in CBL incubated with physiologic concentrations of RA as compared to those without RA.
Conclusion:
The enhancement of IGF expression may be an important pathway for vitamin A to promote immune cellular functions.