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Updated: Oct 3, 2026
Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection
Published on: August 11, 2023
[Localization of endothelin-1 in biopsies from oral submucous fibrosis: immunoelectron microscopic study]
1Department of Stomatology, Xiangya Hospital, Hunan Medical University, Changsha 410008, China.
Objective:
To determine the endothelin-1 (ET-1) immunoreactivity in biopsies from human oral submucous fibrosis (OSF) and to investigate the relation between ET-1 and OSF.
Methods:
The localization of ET-1 in biopsies from OSF was observed with immunoelectron microscopic techniques.
Results:
High-density deposits existed predominantly in the cytoplasm of epithelial cells, fibroblasts and endothelial cells, but not found in the biopsies of normal controls. In the biopsies of OSF, high-density granules were lined on the cell membrane, possibly on the rough endoplasmic reticulum and the mitochondria of the epithelial cells and of the fibroblasts. But a few granules were seen in the cytoplasm of the endothelial cells of microvesseles.
Conclusion:
ET-1 is one of the pathogenetic factors of OSF.
