Related Experiment Video
Updated: Aug 2, 2026
Generation and Culturing of Primary Human Keratinocytes from Adult Skin
Published on: December 22, 2017
In-vitro cultivation of normal human oral keratinocytes
1Department of Oral Medicine, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Objective:
To establish a method for culturing normal human oral keratinocytes.
Methods:
Specimens obtained from healthy humans undergoing oral surgery were dissociated into single cell suspensions by dispase and trypsin. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope. Cytokeratins were shown by immunohistochemistry.
Results:
Cells could be maintained in culture up to 4-5 passages or 30-50 days. Electron microscope revealed that there were desmosomes and tonofibrils in the oral keratinocytes. The cells showed positive staining for cytokeratin antibody.
Conclusion:
Human oral keratinocytes have been successfully grown in serial culture.
