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Updated: Aug 9, 2026
Measuring Fast Calcium Fluxes in Cardiomyocytes
Published on: November 29, 2011
Histochemical study of transmyocardial laser channels in dogs
1Department of Cardiology, Third Hospital, Beijing University Medical School, Beijing 100083, China. leilili@china.com
Objective:
Laser-induced myocardial injury was investigated in a canine transmyocardial laser revascularization model.
Methods:
A total of 23 channels were created in the left ventricles of 5 animals with a CO2 laser. Four animals were sacrificed immediately and one at week 2. Lactate dehydrogenase and succinate dehydrogenase in channel regions were examined with a Nitro-BT method staining.
Results:
Immediately after the procedure, laser channels and surrounding injured myocardium could be stratified from the center to the border into 5 concentric zones, i.e., vaporized zone, carbonized zone, enzyme-disappeared zone, enzyme-decreased zone and enzyme-concentrated zone. Two weeks later, the phenomenon disappeared.
Conclusions:
Immediately after transmyocardial revascularization, there is a ring of enzyme-concentrated zone at the outermost region of injured myocardium. Two weeks later, enzyme activities of reversibly injured myocytes appear to have recovered.
