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Activation of NF-kappa B in bronchial epithelial cells from children with asthma

S Zhao1, Y Qi, X Liu

  • 1Pulmonary Function Laboratory, Beijing Institute of Pediatric Research, Beijing 100045, China.

Chinese Medical Journal
|January 10, 2002
PubMed

Insights

Nuclear factor-kappa B (NF-kappa B) is activated in the airway epithelial cells of children with asthma. This activation may drive airway inflammation and the expression of inflammatory genes in pediatric asthma.

Area of Science:

  • Immunology
  • Pulmonology
  • Molecular Biology

Background:

  • Asthma is a chronic inflammatory airway disease.
  • The role of specific transcription factors in pediatric asthma pathogenesis is under investigation.

Purpose of the Study:

  • To investigate nuclear factor-kappa B (NF-kappa B) activation in epithelial cells of children with asthma.
  • To elucidate the potential role of NF-kappa B in asthma-related airway inflammation.

Main Methods:

  • Bronchial biopsies were collected from children with asthma and healthy controls.
  • Immunohistochemistry was used to detect NF-kappa B expression.
  • Electrophoretic mobility shift assay (EMSA) measured NF-kappa B DNA binding activity.

Main Results:

  • NF-kappa B nuclear expression was detected in epithelial cells of asthmatic children.
  • NF-kappa B DNA binding was present in asthmatic subjects but absent in controls.
  • These findings indicate increased NF-kappa B activity in pediatric asthma.

Conclusions:

  • NF-kappa B is activated in airway epithelial cells of children with asthma.
  • Activated NF-kappa B may contribute to airway inflammation by upregulating inflammatory genes.
  • Targeting NF-kappa B could be a therapeutic strategy for pediatric asthma.
Abstract

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