Related Experiment Video
Updated: Oct 3, 2026
Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells
Published on: March 7, 2025
[Antitumor effect of radiation combined with tumor draining lymphocytes on human ACC-M cell in vitro]
1Department of Oral Maxillofacial Surgery, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Objective:
To find whether there is any synergistic effect of radiation combined with interleukin-2(IL-2) activated tumor draining lymph nodes lymphocytes (DNL) from oral-carcinoma patients on high-lung metastatic salivary adenoid cystic carcinoma cell line(ACC-M).
Methods:
Colony-forming test was used to investigate antitumor effect and analyzed using linear-quadratic(LQ) equation and single hit multi targets equation.
Results:
The ratio of effect to targets was 25:1. The cytotoxicity of DNL was 49.06%. Radiation combined with DNL showed higher antitumor activity compared with radiation alone, alpha value, Dq and S2 were 0.7688 and 0.342 0; 1.5901 and 0.5995; 0.4481 and 0.1135 respectively(P < 0.05).
Conclusions:
It indicates that in initial region of survival curve, DNL significantly increased sublethal damage on ACC-M.
