Related Experiment Video
Updated: Oct 3, 2026
Mechanical Stimulation of Chondrocyte-agarose Hydrogels
Published on: October 27, 2012
[A quick way in isolation and amplification of mandibular condylar cartilage cell in vitro]
1Department of Radiology, School of Stomatology, Beijing Medical University, Beijing 100081, China.
Objective:
To establish a quick way in acquiring well differentiated mandibular condylar cartilage (MCC) cells with high viability in large scale.
Methods:
Japan white rabbit MCC cells were harvested by enzymatic method. They were grown in a modified bioreactor culture system, which contained the cytodex-3 micro-carriers in the culture medium. Kinetic growth of MCC cells on DEAE-dextran micro-carrier was observed under phase contrast microscope and environmental scanning microscope respectively.
Results:
MCC cells attached rapidly to the surface of micro-carriers, but their spreading was slow. A quick growth of these cells was observed when they fully spread onto the micro-carrier. The number of MCC cells increased 16.2 times compared with that of plating.
Conclusions:
Micro-carrier culture of MCC cells can yield a large quantity of cells within a short period of time that will be of benefit in banking MCC cells for reconstruction of impaired cartilage.