Related Experiment Video
Updated: Aug 2, 2026
Generation and Culturing of Primary Human Keratinocytes from Adult Skin
Published on: December 22, 2017
[Serial cultivation of normal human oral keratinocytes]
1Department of Oral Medicine, School of Stomatology, Shanghai Second Medical University, Shanghan 200011, China.
Objective:
To establish a method of culturing normal human oral keratinocytes.
Methods:
Specimens obtained from healthy humans undergoing oral surgery were dissociated by Dispase and trypsin into a single cell suspension. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope (EM). Cytokeratins were showed by immunohistochemistry.
Results:
The cells could be maintained in culture up to 4-5 passages, or 30-50 days. EM revealed that there were desmosomes and tonofibrils in the culture cells. The cells showed positive staining for cytokeratin antibody.
Conclusions:
The culture technique for growing normal human oral keratinocytes has been established.
