Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Matching peptide mass spectra to EST and genomic DNA databases.

J S Choudhary1, W P Blackstock, D M Creasy

  • 1Cell Mapping Project, GlaxoSmithKline R&D, Stevenage, UK.

Trends in Biotechnology
|January 10, 2002
PubMed
Summary

Mass spectrometry data aids protein identification by searching molecular sequence databases. This method can also analyze genomic sequences to confirm gene predictions and discover new genes and splicing patterns.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reduced Palmitoylation of SQSTM1/p62 in Huntington Disease Is Associated With Impaired Autophagy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

Temperature-based phenology model for predicting the present and future establishment and distribution of recently invasive <i>Spodoptera frugiperda</i> (J. E. Smith) in India.

Bulletin of entomological research·2021
Same author

Heavy Metals Scavenging Potential of <i>Trichoderma asperellum</i> and <i>Hypocrea nigricans</i> Isolated from Acid Soil of Jharkhand.

Indian journal of microbiology·2019
Same author

Population genetic structure of cotton pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) using mitochondrial cytochrome oxidase I (COI) gene sequences from India.

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis·2016
Same author

Testicular and epididymal toxicity: Pathogenesis and potential mechanisms of toxicity.

Spermatogenesis·2015
Same author

Comparison of urinary creatine with other biomarkers for the detection of 2-methoxyethanol-induced testicular damage.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals·2013

Area of Science:

  • Proteomics
  • Genomics
  • Bioinformatics

Background:

  • Mass spectrometry is a standard technique for protein identification using molecular sequence databases.
  • Extending this approach to genomic sequences offers new avenues for biological discovery.

Purpose of the Study:

  • To explore the application of mass spectrometry data in analyzing raw genomic sequences.
  • To demonstrate the potential for confirming and correcting predicted coding sequences.
  • To highlight the capability of identifying novel genes and understanding splicing patterns.

Main Methods:

  • Utilizing mass spectrometry data.
  • Searching against molecular sequence databases.
  • Applying the technique to raw genomic sequences.

Related Experiment Videos

Main Results:

  • Experimental confirmation and correction of predicted coding sequences.
  • Identification of potential novel genes.
  • Elucidation of complex splicing patterns.

Conclusions:

  • Mass spectrometry-based searching of genomic sequences is a powerful tool beyond protein identification.
  • This approach validates genomic annotations and uncovers new genetic information.
  • It offers significant potential for advancing our understanding of gene structure and function.