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Related Experiment Videos

Lead-stimulated p38MAPK-dependent Hsp27 phosphorylation.

Rodrigo B Leal1, Fabiano M Cordova, Lynn Herd

  • 1Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, 88040-900, Brazil. rbleal@yahoo.com.br

Toxicology and Applied Pharmacology
|January 10, 2002
PubMed
Summary

Lead (Pb2+) exposure increases the phosphorylation of heat-shock protein 27 kDa (Hsp27) in neural cells. This occurs through the activation of the p38 mitogen-activated protein kinase (MAPK) pathway, revealing a novel mechanism of lead toxicity.

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Area of Science:

  • Neurobiology
  • Toxicology
  • Cellular Signaling

Background:

  • Lead (Pb2+) is a cytotoxic metal ion with an unestablished mechanism of action.
  • Pb2+ is known to interact with signaling molecules, suggesting a role in cellular communication.

Purpose of the Study:

  • To investigate the effect of Pb2+ on protein phosphorylation in bovine adrenal chromaffin cells and human SH SY5Y cells.
  • To elucidate the specific proteins and signaling pathways affected by lead exposure.

Main Methods:

  • Cells were incubated with radioactive phosphate (32P(i)) in the presence of varying Pb2+ concentrations.
  • Proteins were separated using two-dimensional polyacrylamide gel electrophoresis (2D PAGE).
  • Protein identification involved immunoblotting and amino acid sequencing; pathway analysis used a specific p38MAPK inhibitor.

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Main Results:

  • Pb2+ exposure significantly increased the phosphorylation of several proteins, notably three isoforms of heat-shock protein 27 kDa (Hsp27).
  • Pb2+ also increased the phosphorylation of p38 mitogen-activated protein kinase (p38MAPK).
  • Inhibition of p38MAPK blocked the Pb2+-induced phosphorylation of Hsp27, indicating pathway involvement.

Conclusions:

  • Pb2+ modulates the phosphorylation state of Hsp27 in neural cells.
  • This modulation occurs via the activation of the p38MAPK signaling pathway.
  • This study provides the first evidence of Pb2+ affecting Hsp27 phosphorylation through p38MAPK activation.