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Mannose-receptor positive and negative mouse macrophages differ in their susceptibility to infection by Chlamydia
Cho-chou Kuo1, Mirja Puolakkainen, Tsun-Mei Lin
1Department of Pathobiology, University of Washington, Seattle, Washington, Washington 98195, USA. cckuo@u.washington.edu
Abstract:
It has been shown that N-linked high mannose type oligosaccharides competitively inhibits attachment to and infectivity of chlamydiae in HeLa cells. To further study whether mannose moieties are involved in the infectivity of chlamydiae, the susceptibility of mannose-receptor negative J774A and positive J774E mouse macrophages to Chlamydia trachomatis, Chlamydia psittaci and Chlamydia pneumoniae was evaluated. C. trachomatis infected mannose-receptor positive cells better than mannose-receptor negative cells. C. psittaci infected both mannose-receptor negative and positive cells equally well, while C. pneumoniae infected mannose-receptor negative cells better than mannose-receptor positive cells. Further studies using this system may provide insight into the role of mannose-receptor in attachment, entry and survival of chlamydiae in macrophages.
Insights
Mannose receptors influence chlamydiae infection differently depending on the species. Chlamydia trachomatis prefers mannose-receptor positive cells, while Chlamydia pneumoniae prefers negative cells, indicating varied host cell interactions.
Area of Science:
- Immunology
- Microbiology
- Cell Biology
Background:
- N-linked high mannose oligosaccharides inhibit chlamydiae attachment and infectivity in HeLa cells.
- Mannose moieties may play a role in chlamydiae infectivity, necessitating further investigation.
Purpose of the Study:
- To investigate the involvement of mannose moieties in chlamydiae infectivity.
- To evaluate the susceptibility of mannose-receptor negative and positive mouse macrophages to different Chlamydia species.
Main Methods:
- Comparison of Chlamydia trachomatis, Chlamydia psittaci, and Chlamydia pneumoniae infection rates in J774A (mannose-receptor negative) and J774E (mannose-receptor positive) mouse macrophages.
- Utilizing a macrophage model to assess chlamydial entry and survival based on mannose receptor expression.
Main Results:
- Chlamydia trachomatis demonstrated enhanced infection of mannose-receptor positive J774E cells compared to J774A cells.
- Chlamydia psittaci infected both J774A and J774E cells with equal efficiency.
- Chlamydia pneumoniae showed a preference for mannose-receptor negative J774A cells over J774E cells.
Conclusions:
- The role of mannose receptors in chlamydial infection varies significantly among different Chlamydia species.
- This study provides a model system to explore the specific mechanisms of mannose receptor involvement in chlamydial attachment, entry, and intracellular survival within macrophages.