Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 24, 2026

Synthesis of High Purity Nonsymmetric Dialkylphosphinic Acid Extractants
12:06

Synthesis of High Purity Nonsymmetric Dialkylphosphinic Acid Extractants

Published on: October 19, 2017

Extraction and purification of squalene from amaranthus grain.

Han-Ping He1, Yizhong Cai, Mei Sun

  • 1Department of Botany and Department of Zoology, The University of Hong Kong, Pokfulam Road, Hong Kong.

Journal of Agricultural and Food Chemistry
|January 10, 2002
PubMed
Summary

Grain amaranth offers a plant-based source of squalene, a valuable compound. Researchers developed a method to extract and purify squalene from amaranth oil, achieving high purity and yield.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimization of Reaction Conditions to Control Physicochemical Properties of Octenyl Succinic Anhydride Modified Normal Maize Starch.

Foods (Basel, Switzerland)·2026
Same author

Mechanism of α-amylase hydrolysis in maize and potato starches: Insight into outer shells and inner blocklets.

Food chemistry·2026
Same author

Distribution of acetyl groups alters the properties of acetylated starch.

Carbohydrate polymers·2026
Same author

Palliative Effects of Phenolic, Polysaccharide, and Lipid Extracts of Hulless Barley Grass on Acute Ulcerative Colitis.

Molecular nutrition & food research·2026
Same author

Label-Free Proteomics of Starch Granule-Associated Proteins Reveals Starch Molecular Modification during Rice Grain Filling.

Journal of agricultural and food chemistry·2026
Same author

Anti-dyspeptic effects and comparative structural analysis of polysaccharides from hulled and hulless barley-based Hordei Fructus Germinatus.

Carbohydrate polymers·2026

Area of Science:

  • Agricultural Science
  • Biochemistry
  • Nutritional Science

Background:

  • Marine animals are a primary source of squalene.
  • Grain amaranth is explored as a potential alternative natural source of squalene.
  • Understanding amaranth's oil and squalene composition is crucial for its utilization.

Purpose of the Study:

  • To determine oil and squalene content in grain amaranth genotypes.
  • To analyze the fatty acid profiles of amaranth oil.
  • To develop an efficient method for squalene isolation and purification from amaranth oil.

Main Methods:

  • Analysis of oil content, squalene concentration, and fatty acid profiles in 11 amaranth genotypes.
  • Development of a squalene isolation technique involving saponification and silica gel column chromatography.

More Related Videos

Employing Pressurized Hot Water Extraction (PHWE) to Explore Natural Products Chemistry in the Undergraduate Laboratory
09:48

Employing Pressurized Hot Water Extraction (PHWE) to Explore Natural Products Chemistry in the Undergraduate Laboratory

Published on: November 7, 2018

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
10:24

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds

Published on: April 19, 2024

Related Experiment Videos

Last Updated: Jun 24, 2026

Synthesis of High Purity Nonsymmetric Dialkylphosphinic Acid Extractants
12:06

Synthesis of High Purity Nonsymmetric Dialkylphosphinic Acid Extractants

Published on: October 19, 2017

Employing Pressurized Hot Water Extraction (PHWE) to Explore Natural Products Chemistry in the Undergraduate Laboratory
09:48

Employing Pressurized Hot Water Extraction (PHWE) to Explore Natural Products Chemistry in the Undergraduate Laboratory

Published on: November 7, 2018

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
10:24

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds

Published on: April 19, 2024

  • Structural confirmation of purified squalene using UV-Vis spectroscopy and Nuclear Magnetic Resonance (NMR).

    • Main Results:

    • Grain amaranth exhibited low oil content (5.1-7.7%) but high squalene concentrations (3.6-6.1% of total lipids).
    • Major fatty acids identified were palmitic, oleic, and linoleic acids, with high unsaturation.
    • A novel purification method yielded a 94% squalene concentrate with 90% yield and up to 98% purity in fractions.

    Conclusions:

    • Grain amaranth is a viable alternative source for natural squalene.
    • The developed method efficiently isolates high-purity squalene from amaranth oil.
    • Amaranth oil's fatty acid profile indicates significant unsaturation.