[Influence of staphylococcal enterotoxin A on immunogenicity of hepatocellular carcinoma cells]

To transduce the gene of staphylococcal enterotoxin A (SEA) into a HCC cell line HHCC and to make the cells to express the molecules of SEA for the purpose of improving the immunogenicity and inducing immune rejection of HCC.

Construct retroviral vector containing the gene of SEA and transduce it into HHCC cells. Then the integration, transcription and expression of recombinant were analyzed. At last cytotoxicity test of peripheral blood mixed lymphocytes was carried out.

We obtained the retroviral vector pLXSN-SEA, transduced into HHCC cells and obtained the HCC cell line that expressed SEA toxin protein. The content of SEA in supernatant was at the level of pg. The cytotoxicity of HHCC transduced with SEA by T lymphocytes was 45.6%, which was higher than that of HHCC (20.7%). The Km value of T lymphocytes against HHCC transduced with SEA and without SEA was 5.18 x 10(4) and 2.92 x 10(5), separately. The difference had statistical significance.

After transducing the SEA gene, although tiny quantity of expression is detected, the cytotoxicity assay proves that the expression of SEA lead to a robust immune response.