Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Regulating sister chromatid separation by separase phosphorylation.

Koji Nagao1, Mitsuhiro Yanagida

  • 1Department of Biophysics, Graduate School of Science, Kyoto University, 606-8502 Kyoto, Japan.

Developmental Cell
|January 10, 2002
PubMed
Summary

Researchers discovered a new way to control separase, a key protein in cell division. This regulation occurs through separase phosphorylation, a process influenced by CDC2, adding to the known securin inhibition pathway.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ozonated Olive Oil Attenuates Hepatic Steatosis in Obese db/db Mice: Evidence for Triacylglycerol-derived Ozonides as Key Bioactive Species.

Journal of oleo science·2026
Same author

The metabolites for muscle and osteoclast activity are indicators of femoral neck osteoporosis.

Scientific reports·2026
Same author

Author Correction: RNaseH2A downregulation drives inflammatory gene expression via genomic DNA fragmentation in senescent and cancer cells.

Communications biology·2025
Same author

scRepli-RamDA-seq: a multi-omics technology enabling the analysis of gene expression dynamics during S-phase.

Nature communications·2025
Same author

Metabolomic Analysis of Fermented Nori Powders: Divergence of Betaine Structural Analogs Production by Three Types of <i>koji</i> Fungal Fermentation.

Molecules (Basel, Switzerland)·2025
Same author

S. pombe Mis4 is required for exit from G0 as it is necessary for full nuclear separation during the subsequent M phase.

Journal of cell science·2025

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Separase is a critical protease for cell division, ensuring accurate chromosome segregation.
  • Separase activity is primarily regulated by its inhibitor, securin.
  • Understanding alternative regulatory mechanisms is crucial for comprehending cell cycle control.

Purpose of the Study:

  • To identify and characterize novel regulatory pathways for separase.
  • To investigate the role of post-translational modifications in separase regulation.
  • To elucidate the relationship between separase, securin, and other cell cycle regulators.

Main Methods:

  • The study employed biochemical assays to analyze separase activity.
  • Phosphorylation sites on separase were identified using mass spectrometry.

Related Experiment Videos

  • Experiments involving the kinase CDC2 were performed to assess its effect on separase.

    • Main Results:

    • A second, independent mechanism regulating separase was identified.
    • Separase phosphorylation was demonstrated to be a key regulatory event.
    • This phosphorylation pathway was shown to be dependent on the activity of CDC2.

    Conclusions:

    • Separase regulation is more complex than previously understood, involving both securin binding and phosphorylation.
    • CDC2-dependent phosphorylation represents a significant new pathway controlling separase activity.
    • These findings offer new insights into the precise control of cell division and chromosome segregation.