Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Embryonic expression of an Nkx2-5/Cre gene using ROSA26 reporter mice.

K A Moses¹, F DeMayo, R M Braun

¹Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.

Genesis (New York, N.Y. : 2000)

|January 10, 2002

Summary

Researchers developed a new Nkx2-5/Cre mouse model to track gene activity during heart development. This model uses beta-galactosidase to visualize Nkx2-5 expression in early embryonic tissues, aiding cardiogenesis research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reflectance confocal microscopy - a non-invasive tool for monitoring systemic treatment response in stage III unresectable primary scalp melanoma.

Journal of the European Academy of Dermatology and Venereology : JEADV·2022

Same author

Successful treatment of eruptive keratoacanthomas with actitretin for patients on checkpoint inhibitor immunotherapy.

Journal of the European Academy of Dermatology and Venereology : JEADV·2022

Same author

The impact of sociodemographic factors and PSA screening among low-income Black and White men: data from the Southern Community Cohort Study.

Prostate cancer and prostatic diseases·2017

Same author

Uterine RAC1 via Pak1-ERM signaling directs normal luminal epithelial integrity conducive to on-time embryo implantation in mice.

Cell death and differentiation·2015

Same author

Emerging association between androgen deprivation therapy and male meningioma: significant expression of luteinizing hormone-releasing hormone receptor in male meningioma.

Prostate cancer and prostatic diseases·2013

Same author

High level of dioxin-TEQ in tissue is associated with Agent Orange exposure but not with biochemical recurrence after radical prostatectomy.

Prostate cancer and prostatic diseases·2013

Area of Science:

Developmental Biology
Genetics
Embryology

Background:

Nkx2-5 is a crucial early marker for cardiac development in vertebrates.
Understanding gene expression patterns is vital for studying developmental processes.

Purpose of the Study:

To create a genetic model for tracking Nkx2-5 gene activity during embryonic development.
To establish a tool for investigating the role of Nkx2-5 in cardiogenesis and other developmental pathways.

Main Methods:

Homologous recombination was used to knock in the Cre recombinase gene into the Nkx2-5 locus.
Nkx2-5/Cre mice were crossed with ROSA26 reporter (R26R) mice to visualize gene activity via beta-galactosidase (beta-gal) staining.
beta-gal activity was analyzed in embryos at various developmental stages.

Related Experiment Videos

Main Results:

beta-gal activity was detected in the early cardiac crescent, looping heart tube cardiomyocytes, and pharyngeal arch epithelium.
Later embryonic stages (10.5-13.5 dpc) showed beta-gal activity in the stomach, spleen, tongue dorsum, and developing tooth primordium.
The Nkx2-5/Cre mouse model successfully reports Nkx2-5 expression patterns.

Conclusions:

The Nkx2-5/Cre mouse model is a valuable genetic resource for studying cardiogenesis.
This model facilitates the investigation of gene function in developmental processes involving Nkx2-5.
It enables the study of loxP-manipulated genetic targets in heart development and beyond.