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[Effect of tongbiling on synoviocyte function in adjuvant arthritis rats]
Objective:
To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblast and interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha) and postaglandin E2(PGE2) secretion by synoviocytes in adjuvant arthritis (AA) rats.
Methods:
Synovial fibroblasts was derived from culture of tissue piece. The effect of primary synoviocyte culture supernatants on the fibroblast proliferation were assayed and IL-1, TNF-alpha bioactivity and PGE2 content of supernatants of cultured synoviocytes were measured.
Results:
TBL could inhibit significantly the synovial fibroblast proliferation (P < 0.001), and down-regulated IL-1, TNF-alpha and PGE2 productions (P < 0.01); indomethacin could promote obviously the synovial fibroblast proliferation (P < 0.001). It inhibited significantly PGE2 production, but further up-regulated the IL-1 and TNF-alpha secretion by synoviocytes (P < 0.01).
Conclusion:
The therapeutical effect of TBL on AA might be associated with its down-regulating the secretory function of synoviocyte, then restoring the abnormal proliferation of fibroblast to normal levels.