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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
Published on: February 21, 2014
[Expression of estrogen receptor beta mRNA in endometrial carcinoma and its significance]
1Department of Obstetrics and Gynecology, First Hospital, Peking University, Beijing 100034, China.
Objective:
To investigate the expression of estrogen receptor beta (ER beta) mRNA and its significance in endometrial carcinoma.
Methods:
Thirty-six normal and 45 cancer tissue samples of endometrium were obtained, and the RNA was isolated from the tissues by the acid guanidium thiocyanate-phenol-chloroform extraction method. Reverse-transcription polymerase chain reaction (RT-PCR) was used to amplify the ER beta exon 5 wild type mRNA (ER beta WT) and splicing variant mRNA (ER beta E5SV). Electrophoresis of polymerase chain reaction product was performed and the ratio of ER beta E5SV/ER beta WT was calculated. The sequencing of ER beta E5SV and ER beta WT was carried out by express DNA sequencer.
Results:
ER beta E5SV and ER beta WT were both expressed in normal endometrium and endometrial cancer tissues. A region of 139 bp from base pair 812 to 950 (the hormone-binding domain) was found to be deleted in ER beta E5SV sequence as compared to ER beta WT. The quantity of ER beta E5SV was more than that of ER beta WT in every normal endometrium, and was less in every endometrial cancer, the ratio of ER beta E5SV/ER beta WT was 2.47 +/- 0.99 in normal endometrium, and 0.55 +/- 0.12 in endometrial cancer (P < 0.0001). The ratio of ER beta E5SV/ER beta WT decreased with the grade from 1 to 3 (P < 0.01).
Conclusion:
Overexpression of ER beta WT relative to ER beta E5SV may correlate with the genesis of endometrial carcinoma.
