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[Relationship between follicular development and insulin like growth factor I receptor in stimulated cycles]
1Department of Gynecology, Affiliated Obstetrics and Gynecology Hospital, Faculty of Medicine, Zhejiang University, Hangzhou 310006, China.
Objective:
To investigate the number of follicles and insulin-like growth factor I receptor (IGF-I R) in stimulated cycles.
Method:
IGF-I R mRNA and IGF-I R in granulosa cells obtained during oocyte retrieval were respectively measured by reverse transcript polymerase chain reaction (RT-PCR) and Western blot technique.
Results:
The expression of IGF-I R mRNA in poor responders (follicles < or = 3) was much lower than in normal responders (follicles 4-13) and high responders (follicles > or = 14) (1.70 +/- 0.23, 2.92 +/- 0.49, 4.22 +/- 1.50 respectively). Similar results were obtained for IGF-I R (1.32 +/- 0.31, 2.01 +/- 0.72, 4.39 +/- 2.31 respectively).
Conclusion:
The expression of IGF-I R correlated with the effects of gonadotropin hormone on follicular development.