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Updated: Aug 6, 2026
Bridging the Bio-Electronic Interface with Biofabrication
Published on: June 6, 2012
Immobilization of lactate dehydrogenase on tetraethylorthosilicate-derived sol-gel films for application to lactate
A Chaubey1, M Gerard, V S Singh
1Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, New Delhi, India. achaubey@csnpl.ren.nic.in
Abstract:
Tetraethylorthosilicate (TEOS)-derived sol-gel films were utilized for the immobilization of lactate dehydrogenase (LDH) by physical adsorption and sol-gel/LDH/sol-gel sandwich configuration. An attempt was made to ascertain the optimum pH and temperature for the immobilized LDH. It was shown that TEOS-derived sol-gel films containing physically adsorbed LDH exhibited linearity from 0.5 to 4 mM, whereas those containing LDH in sandwich configuration showed linearity from 0.5 to 3 mM L-lactate. These sol-gel films, immobilized with LDH, were found to be stable for about 4 weeks at 4-10 degrees C.
