Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A synthetic pentasaccharide with GTPase activity.

D Solomon1, M Fridman, J Zhang

  • 1Department of Chemistry, Institute of Catalysis Science and Technology, Technion - Israel Institute of Technology, Haifa 32000, Israel.

Organic Letters
|January 11, 2002
PubMed
Summary

Researchers developed a synthetic pentasaccharide that significantly accelerates GTP hydrolysis to GDP and orthophosphate. This novel molecule demonstrates remarkable rate enhancement and specificity, offering potential applications in biochemical research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A diagnostic certainty score to support improvements in culture confirmation of TB.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2025
Same author

Life-cycle assessment of oil recovery using dimethyl ether produced from green hydrogen and captured CO<sub>2</sub>.

Scientific reports·2025
Same author

Meat quality of Sasso T44 and Koekoek cockerels exposed to temperature variation with supplementary coriander seed powder.

Tropical animal health and production·2022
Same author

How do we measure unmet need within sexual and reproductive health? A systematic review.

Perspectives in public health·2022
Same author

The Role of Sex Work Pay in Moderating the Effect of Mobile Phone Solicitation on Condom Practices: An Analysis of Female Sex Workers in India.

HSOA journal of AIDS clinical research & STDs·2017
Same author

Abstracts from Hydrocephalus 2016.

Fluids and barriers of the CNS·2017

Area of Science:

  • Synthetic chemistry
  • Biochemistry
  • Enzyme kinetics

Background:

  • Guanosine triphosphate (GTP) hydrolysis is crucial for various cellular processes.
  • Specific catalysts are needed to efficiently study GTPase activity.
  • Existing methods for GTP hydrolysis lack sufficient rate enhancement and specificity.

Purpose of the Study:

  • To design and synthesize a novel pentasaccharide molecule.
  • To evaluate the catalytic efficiency and specificity of the synthetic pentasaccharide for GTP hydrolysis.
  • To report the first example of a synthetic pentasaccharide acting as a GTPase accelerator.

Main Methods:

  • Chemical synthesis of a pentasaccharide (compound 1).
  • Assay development for monitoring GTP hydrolysis.

Related Experiment Videos

  • Kinetic studies to determine rate enhancement and specificity under defined conditions (pH 7.1, 50°C).

    • Main Results:

    • Successful synthesis and characterization of the pentasaccharide.
    • Demonstrated marked rate enhancement for GTP hydrolysis to GDP and orthophosphate (OP).
    • Achieved approximately 500-fold rate enhancement at specific substrate and cofactor concentrations (GTP/1 = 3.6, GTP/Mg(2+) = 1).

    Conclusions:

    • The synthetic pentasaccharide is the first example of such a molecule to show significant rate enhancement for GTP hydrolysis.
    • This pentasaccharide acts as a potent and specific catalyst for GTP to GDP and OP conversion.
    • The findings open new avenues for studying GTP-dependent biological processes using synthetic catalysts.