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[Transforming activity and the oncogenicity of simian adenovirus SA7 DNA]

T I Ponomareva, N M Chaplygina, B S Naroditskiĭ

    Biulleten' Eksperimental'Noi Biologii I Meditsiny
    |December 1, 1979
    PubMed

    Insights

    Simian adenovirus SA7 DNA transforms cells and causes tumors. Specific DNA fragments generated by Bam HI retain transforming activity, while SalI fragments show oncogenicity in hamsters, indicating distinct viral gene functions.

    Area of Science:

    • Molecular biology
    • Virology
    • Oncology

    Background:

    • Simian adenovirus SA7 (AdSA7) is a virus with known transforming and oncogenic potential.
    • Understanding the specific viral DNA regions responsible for these activities is crucial for cancer research.

    Purpose of the Study:

    • To investigate the transforming and oncogenous activity of intact AdSA7 DNA.
    • To determine the biological activity of AdSA7 DNA fragments generated by restriction endonucleases Bam HI and SalI.

    Main Methods:

    • Cell transformation assays using rat kidney cells and embryo fibroblasts.
    • Tumor induction studies in newborn hamsters.
    • DNA restriction endonuclease digestion (Bam HI and SalI).

    Main Results:

    • Intact AdSA7 DNA demonstrated both transforming and oncogenous capabilities.
    • AdSA7 DNA digested with Bam HI retained transforming activity.
    • The DNA fragments produced by SalI digestion were oncogenous in hamsters.

    Conclusions:

    • Specific regions of AdSA7 DNA are responsible for cellular transformation and tumor formation.
    • Bam HI restriction fragments contain genetic information for cell transformation.
    • SalI restriction fragments harbor oncogenic potential, suggesting distinct functional roles for different viral DNA segments.

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