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[Isolation and characterization of Cytophaga lytica lipopolysaccharide]
L D Varbanets1, V A Ivanitsa, O S Brovarskaia
1Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Abstract:
Lipopolysaccharide (LPS) and its structural components: lipid A, O-specific polysaccharide (O-PS) and oligosaccharide core (OG-core) have been isolated from Cytophaga lytica. 3-Oxytetradecanoic (40.8%) and dodecanoic (28.7%) are the predominant fatty acids of lipid A; pentadecanoic (6.8%), 3-oxyhexadecanoic (6.5%) as well as hexadecanoic (5.4%) acids have been found as well. The content of the rest of fatty acids is inconsiderable (2.3 to 0.5%). OG-core contained monosaccharides both typical of the most Gram-negative bacteria (glucose, mannose, galactose, rhamnose, glucosamine) and rarely occurring one-arabinose. O-PS is represented by glucose, mannose, rhamnose, glucosamine, galactosamine as well as unidentified hexosamine.