Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rabies01:28

Rabies

Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Teplizumab (Tzield) for the delay of type 1 diabetes.

JAAPA : official journal of the American Academy of Physician Assistants·2026
Same author

AAV-mediated gene transfer of a novel microdystrophin ameliorates pathology and enhances muscle function in a mouse model of DMD.

Molecular therapy. Nucleic acids·2026
Same author

Improving angiogenesis ameliorates the efficacy of ASO-based exon skipping for the treatment of Duchenne muscular dystrophy.

Molecular therapy. Nucleic acids·2026
Same author

How to do (or not to do)… asset mapping in community health.

Health policy and planning·2026
Same author

Systemic distribution of tricyclo-DNA antisense oligonucleotide following intratracheal instillation in the mouse.

NAR molecular medicine·2026
Same author

Epidural Anesthesia in a Patient With Turner Syndrome: A Case Report.

Cureus·2025

Related Experiment Video

Updated: Jul 15, 2026

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
14:55

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors

Published on: December 3, 2013

Neurotropism and retrograde axonal transport of a canine adenoviral vector: a tool for targeting key structures

Elise Peltékian1, Luis Garcia, Olivier Danos

  • 1Genethon III-Centre National de la Recherche Scientifique URA 1923, 1 bis rue de l'Internationale, 91002 Evry, France.

Molecular Therapy : the Journal of the American Society of Gene Therapy
|January 12, 2002
PubMed
Summary

Canine adenovirus type 2 (CAV2) vectors show high affinity for neuronal cells, enabling selective gene transfer. These vectors are rapidly transported retrogradely, offering potential for targeting neurodegenerative diseases like Alzheimer's and Parkinson's.

More Related Videos

Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery
07:27

Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery

Published on: June 30, 2021

Widespread Transduction of Mouse Neocortical Neurons by Subarachnoid Injection of AAV2
07:40

Widespread Transduction of Mouse Neocortical Neurons by Subarachnoid Injection of AAV2

Published on: May 23, 2025

Related Experiment Videos

Last Updated: Jul 15, 2026

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
14:55

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors

Published on: December 3, 2013

Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery
07:27

Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery

Published on: June 30, 2021

Widespread Transduction of Mouse Neocortical Neurons by Subarachnoid Injection of AAV2
07:40

Widespread Transduction of Mouse Neocortical Neurons by Subarachnoid Injection of AAV2

Published on: May 23, 2025

Area of Science:

  • Neuroscience
  • Virology
  • Gene Therapy

Background:

  • Viral tropism dictates virus-host cell interactions.
  • Adenovirus type 5 (Ad5) vectors transduce both neurons and glial cells.
  • Canine adenovirus type 2 (CAV2) naturally targets respiratory epithelium.

Purpose of the Study:

  • To investigate the neuroanatomical tropism of CAV2 vectors.
  • To evaluate CAV2 vector uptake and transport in rat brains.
  • To assess the potential of CAV2 vectors for targeting neurodegenerative disease-affected brain regions.

Main Methods:

  • Intracerebral injections of CAV2 and Ad5 vectors in rat brains.
  • In vitro studies using Cy3-labeled CAV2 particles to assess neuronal affinity.
  • In vivo tracking of labeled CAV2 particles via retrograde axonal transport.

Main Results:

  • CAV2 vectors selectively transduced neuronal cells, unlike Ad5 vectors.
  • CAV2 demonstrated high affinity for neuronal processes in vitro.
  • Rapid retrograde axonal transport of CAV2 was observed in vivo, reaching the entorhinal cortex and substantia nigra.
  • Massive transduction of dopaminergic neurons in the substantia nigra was achieved after striatal injections.

Conclusions:

  • CAV2 vectors exhibit selective neuronal tropism and efficient retrograde transport.
  • This vector system holds promise for targeting specific neuronal populations, including those affected in Alzheimer's and Parkinson's diseases.
  • CAV2 vectors offer a novel tool for gene therapy in neurodegenerative disorders.