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Updated: Jul 12, 2026

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
Peptide analog of fibronectin that inhibits cell migration and ERK 1/2 activity
B Szaniawska1, H Trembacz, J Miloszewska
1Department of Cell Biology, The Maria Sklodowska -Curie Memorial Cancer Center, WK Roentgen Str. 5, 02-781, Warsaw, Poland.
Abstract:
Two analogs of the peptide mimicking the 1977-1991 C- terminal part of fibronectin have been synthesized and tested. AWLI simulated human fibronectin fragment 1977-1991, whereas AWLII hybridized to both RGD and 1977-1991 fragments. AWLI and AWLII peptides inhibited the migration of the ovarian carcinoma cell line OVP10 regardless of the presence RGD. AWLI peptide inhibited spontaneous and fibronectin-activated cell migration and ERK1/2 activity. Neither AWLI nor fibronectin induced changes in FAK proteins, as could be judged from Western blots. In conclusion, it seems that the C-terminal fragment of fibronectin inhibits ERK1/2-dependent (random) migration of ovarian carcinoma cells.
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