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Oligonucleotide lipoplexes: the influence of oligonucleotide composition on complexation
V M Meidan1, J Glezer, N Amariglio
1Advanced Technology Center, Chaim Sheba Medical Center, Tel Hashomer, Israel.
Biochimica Et Biophysica Acta
|January 12, 2002
Summary
Oligonucleotide sequence significantly impacts lipoplex properties. Different nucleotide bases and sequences alter how oligonucleotides complex with DOTAP liposomes, affecting charge and potentially transfection efficiency.
Area of Science:
- Biochemistry
- Materials Science
- Nanotechnology
Background:
- Oligonucleotide lipoplexes are crucial for gene delivery.
- Physicochemical properties of lipoplexes are not well understood concerning oligonucleotide sequence.
Purpose of the Study:
- To investigate how oligonucleotide sequence and composition affect lipoplex physicochemical properties.
- To determine if nucleotide base selection influences lipoplex formation and characteristics.
Main Methods:
- Complexation of DOTAP liposomes with various 18-mer phosphorothioate oligonucleotides (homo-oligonucleotides and G3139 variants).
- Analysis of pH and surface potential using heptadecyl-7-hydroxycoumarin fluorescence.
- Particle size distribution analysis via static light scattering.
Main Results:
- Different homo-oligonucleotides exhibited distinct complexation behaviors with DOTAP.
- Polyadenine S-ODN showed lower DOTAP neutralization, resulting in a more positively charged lipoplex.
- Electrostatic differences were less pronounced between lipoplexes of G3139-related oligonucleotides.
Conclusions:
- Nucleotide base selection and sequence are critical variables influencing oligonucleotide lipoplex physicochemical properties.
- These sequence-dependent variations likely impact the transfection competency of lipoplexes.