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A novel enzyme biosensor for steroidal glycoalkaloids detection based on pH-sensitive field effect transistors
Y I Korpan1, V V Volotovsky, C Martelet
1Ukrainian Centre of Biosensors, 54, Volodymyrska str., 01030, Kiev 30, Ukraine.
Bioelectrochemistry (Amsterdam, Netherlands)
|January 12, 2002
Summary
A novel biosensor utilizing pH-Sensitive Field Effect Transistors and butyrylcholinesterase enables sensitive detection of potato glycoalkaloids. This reusable biosensor offers reliable measurements for total glycoalkaloids in a broad concentration range.
Area of Science:
- Biotechnology
- Biosensor Technology
- Analytical Chemistry
Background:
- Steroidal glycoalkaloids, such as alpha-chaconine, alpha-solanine, and solanidine, are toxic compounds found in potatoes.
- Accurate detection of these glycoalkaloids is crucial for food safety and quality control.
- Existing detection methods can be time-consuming or require complex sample preparation.
Purpose of the Study:
- To develop a novel biosensor for the sensitive and selective detection of steroidal glycoalkaloids.
- To utilize pH-Sensitive Field Effect Transistors (pH-FETs) as transducers and immobilised butyrylcholinesterase (BChE) as the biorecognition element.
- To establish a reproducible and reusable biosensing platform for glycoalkaloid analysis.
Main Methods:
- Immobilisation of butyrylcholinesterase onto pH-Sensitive Field Effect Transistors.
- Measurement of glycoalkaloid concentrations using the developed biosensor.
- Evaluation of biosensor performance, including sensitivity, detection limits, reproducibility, and reusability.
- Assessment of sensor stability during storage.
Main Results:
- The biosensor successfully measured total potato glycoalkaloids in the concentration range of 0.5–100 µM.
- Detection limits were determined as 0.5 µM for alpha-chaconine and 2.0 µM for alpha-solanine and solanidine.
- High reproducibility was observed, with relative standard deviations of 1.5% for intra-sensor and 5% for inter-sensor measurements.
- The biosensor demonstrated excellent reusability (over 100 measurements) and stability when stored at 4°C for at least 3 months.
Conclusions:
- The developed biosensor offers a sensitive, reproducible, and reusable platform for detecting steroidal glycoalkaloids.
- The combination of pH-FETs and BChE provides an effective approach for glycoalkaloid monitoring.
- This biosensor has potential applications in food safety analysis and quality control of potato products.