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[Studies on the application of antifreeze proteins in cryopreservation of rice suspension cells].

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The dual effect of antifreeze protein on cryopreservation of rice (Oryza sativa l.) embryogenic suspension cells.

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Pregrowth-desiccation: a simple and efficient procedure for the cryopreservation of rice (Oryza sativa L.)

Y X Zhang1, J H Wang, H W Bian

  • 1College of Life Sciences, Zhejiang University, Hangzhou 310012, China.

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Cryopreservation of rice cells using a pregrowth-desiccation method achieved high survival rates. This simple technique allows cryostored cells to regenerate fertile plants efficiently.

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Area of Science:

  • Plant Biotechnology
  • Cryobiology
  • Cell Biology

Background:

  • Cryopreservation is crucial for preserving plant genetic resources.
  • Developing efficient methods for rice suspension cell cryopreservation is essential for its conservation and research.
  • Traditional cryopreservation methods may not be optimal for all cell types.

Purpose of the Study:

  • To establish a simple and effective cryopreservation protocol for rice embryogenic suspension cells.
  • To evaluate the survival and regenerative capacity of cryopreserved rice cells.
  • To demonstrate the applicability of the pregrowth-desiccation method for rice cell cryopreservation.

Main Methods:

  • Rice embryogenic suspension cells were precultured in specific sucrose and sorbitol media.
  • Cells underwent a controlled air-drying process to achieve a low water content (10%).
  • Desiccated cells were then rapidly frozen in liquid nitrogen.

Main Results:

  • The pregrowth-desiccation procedure resulted in high survival rates: 96+/-6% (TTC assay) and 100% (cell clump regrowth).
  • Cryopreserved cells exhibited rapid revival during recovery culture.
  • The regenerated plants from cryopreserved cells were fertile.

Conclusions:

  • Air-drying, combined with pregrowth, is a simple and efficient method for cryopreserving precultured rice suspension cells.
  • This method overcomes limitations of traditional cryopreservation techniques for these cells.
  • The successful regeneration of fertile plants confirms the viability and utility of this cryopreservation approach.