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Oxygen transfer rate and sophorose lipid production by Candida bombicola
Vladimir Guilmanov1, Alberto Ballistreri, Giuseppe Impallomeni
1Department of Chemistry, Chemical Engineering, and Material Science, Polytechnic University, Brooklyn, NY 11201, USA.
Biotechnology and Bioengineering
|January 15, 2002
Abstract:
Sophorose lipids (SLs) have applications as surfactants and are produced at high levels by several yeasts. We developed a fed-batch shake-flask method for the production of SLs by Candida bombicola ATCC 22214. Optimal aeration, expressed in terms of oxygen transfer rate, was between 50 and 80 mM O(2)/L h(-1) and resulted in maximum values for both volumetric product formation (1-1.5 g/L h(-1)) and SL yield (350 g/L). The lowest aeration levels resulted in the enrichment in saturated fatty acid SLs at the expense of unsaturated fatty acid SLs.