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[Inhibitory effect of panicoin on platelet activation and its mechanism]
Objective:
To explore the inhibitory pathway and the possible mechanism of panicoin on platelet activation.
Methods:
The effect of panicoin on platelet aggregation, thromboxane B2 release, P-selectin (GMP-140) count, levels of cyclic AMP and cyclic GMP induced by ADP, epinephrine, arachidonic acid (AA) or thrombin were observed.
Results:
(1) Panicoin significantly inhibits platelet aggregation induced by ADP, arachidonic acid and epinephrine in a dose-dependent manner; (2) It reduces the P-selectin number on platelet surface which was stimulated by thrombin; (3) It inhibits TXB2 release induced by AA and increases the platelet cyclic AMP level, while cyclic GMP level was not changed.
Conclusion:
Panicoin shows a potent inhibition on platelet activation and antithrombotic effect. The mechanisms seem to be related with the elevation of platelet cyclic AMP.