Conditioned taste aversion to gut distension in rats

The aim of this work was to study the negative effects of the intestinal stimulation by a method that is sensitive to assess internal malaise and discomfort. Effects of volumetric and "isometric" gut distension on the behavior were compared in the small and the large intestines, respectively. Chronically separated (Thiry-Vella) intestinal loops prepared from the upper jejunum and/or from the uppermost segment of the colon were stimulated with a rubber balloon. Conditioned taste aversion (CTA) series were accompanied with recording satiety and aversive behavioral indexes. Both isometric and volumetric stimuli elicited taste aversion. No essential differences between the two intestinal parts were found. Behavioral indices supported intake data: satiety indexes were similar to each other, whereas aversive indexes were high during stimulation and lower during testing. Data were consistent over conditions and time, proving the reliability of the method. Results are compared to earlier taste reactivity records.