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NADPH-dependent L-sorbose reductase is responsible for L-sorbose assimilation in Gluconobacter suboxydans IFO 3291
Masako Shinjoh1, Masaaki Tazoe, Tatsuo Hoshino
1Department of Applied Microbiology, Nippon Roche Research Center, 200 Kajiwara, Kamakura 247, Japan. masako.shinjoh@roche.com
Journal of Bacteriology
|January 16, 2002
Abstract:
The NADPH-dependent L-sorbose reductase (SR) of L-sorbose-producing Gluconobacter suboxydans IFO 3291 contributes to intracellular L-sorbose assimilation. The gene disruptant showed no SR activity and did not assimilate the once-produced L-sorbose, indicating that the SR functions mainly as an L-sorbose-reducing enzyme in vivo and not as a D-sorbitol-oxidizing enzyme.