[The adhesion and seeding of NIH3T3 fibroblast on PHB surfaces]

Biodegradable polymers have been widely used in the field of tissue engineering. The adhesive characteristic of polymer surfaces is critical for cell seeding and growth. The adhesion and growth of NIH3T3 fibroblast on poly-beta-hydroxybutrpic acid (PHB) was studied here, and the cell-surface adhesion strength was measured in a shear flow detachment assay. It showed that the critical shear stress for cell detachment was relatively low on PHB, thus cells tend to aggregate and detach from surfaces. On the other hand, cells tend to spread and formed monolayer on poly-lysine coated PHB surface. When the PHB foams scaffolds were coated by poly-lysine, the seeding percentage of cells was also greatly enhanced.