Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Flow-based microimmunoassay.

M A Hayes1, T N Polson, A N Phayre

  • 1Department of Chemistry and Biochemistry and The Center for Solid State Electronics Research, Arizona State University, Tempe 85287, USA. mhayes@asu.edu

Analytical Chemistry
|January 17, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of a test-and-cull strategy to control bovine paratuberculosis based on the detection of Mycobacterium avium ssp. paratuberculosis DNA using the high-throughput Johne's fecal polymerase chain reaction test.

Journal of dairy science·2025
Same author

Microstructure response of concentrated suspensions to flow reversal.

The European physical journal. E, Soft matter·2025
Same author

Relationship between internal medicine journals' activity on social networks and the citations they receive.

Revista clinica espanola·2021
Same author

Pathologic fracture healing after femoral limb salvage in a dog.

Australian veterinary journal·2019
Same author

Purified Human Pancreatic Islets, CIT Culture Media with Lisofylline or Exenatide.

CellR4-- repair, replacement, regeneration, & reprogramming·2019
Same author

A multicenter, open-label, expanded phase 2 study to evaluate the safety and efficacy of etirinotecan pegol, a polymer conjugate of irinotecan, in women with recurrent platinum-resistant or refractory ovarian cancer.

Gynecologic oncology·2017

This study introduces a microfluidic immunoassay system using magnetic beads for rapid, sensitive detection in small sample volumes. The technology enables quick, high-performance diagnostic tests with minimal reagent use.

Area of Science:

  • Biotechnology and Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Traditional immunoassays often require large sample volumes and longer assay times.
  • Microfluidic systems offer potential for miniaturization and increased assay efficiency.
  • Paramagnetic particles can be manipulated magnetically for controlled assay environments.

Purpose of the Study:

  • To develop and demonstrate a small-volume heterogeneous immunoassay system utilizing microchannels and magnetic manipulation.
  • To achieve high sensitivity and rapid assay times using small paramagnetic particles.
  • To validate the system for detecting clinically relevant biomarkers.

Main Methods:

  • A microfluidic chip was designed to house paramagnetic particles (1-2 µm diameter).

Related Experiment Videos

  • Magnetic fields were used to control particle manipulation and form packed beds within microchannels.
  • Assays involved passing microliter to nanoliter volumes of samples and reagents through the microchannels.

    • Main Results:

    • A direct immunoassay for fluorescein isothiocyanate (FITC) achieved 90% maximum signal in under 3 minutes with <1 µL sample.
    • Heterogeneous sandwich assays for parathyroid hormone (PTH) and interleukin-5 (IL-5) were successfully performed.
    • Both PTH and IL-5 assays demonstrated physiologically relevant sensitivity at approximately µg/L levels.

    Conclusions:

    • The demonstrated microfluidic immunoassay system provides a sensitive and rapid platform for small-volume biological sample analysis.
    • The use of small paramagnetic particles enhances the surface-area-to-volume ratio, crucial for high sensitivity.
    • This technology holds promise for point-of-care diagnostics and high-throughput screening applications.