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Immobilization of biomolecules on polyurethane membrane surfaces
Patrick Reichmuth1, Hans Sigrist, Martin Badertscher
1Institute of Microtechnology (IMT), University of Neuchâtel, Rue Jaquet-Droz 1, CH-2007 Neuchâtel, Switzerland.
Bioconjugate Chemistry
|January 17, 2002
Summary
Researchers biofunctionalized calcium-selective membranes with photoactivatable biotin. This modification enables selective streptavidin binding, enhancing the potential of potentiometric sensors for broader applications.
Area of Science:
- Polymer Science
- Biomaterials Science
- Sensor Technology
Background:
- Lipophilic polymer membranes with binding sites are crucial for potentiometric, amperometric, and optical sensors.
- Surface modification of these membranes is key to expanding their sensing capabilities.
- Calcium-selective membranes are widely utilized in various sensing applications.
Purpose of the Study:
- To biofunctionalize the surface of a calcium-selective membrane.
- To covalently immobilize a photoactivatable biotin derivative onto a polyurethane membrane.
- To demonstrate the selective binding of streptavidin to the modified membrane.
Main Methods:
- Synthesis and immobilization of a photoactivatable biotin derivative.
- Characterization using X-ray photoelectron spectroscopy (XPS) and potentiometric measurements.
- Analysis of surface coverage via autoradiography with [(35)S]-streptavidin.
Main Results:
- Successful covalent immobilization of the photoactivatable biotin derivative on the polyurethane membrane.
- Demonstration of selective streptavidin binding to the photo-cross-linked biotin.
- Quantification of surface coverage using autoradiography.
Conclusions:
- The biofunctional modification approach is effective for creating selective binding sites on sensor membranes.
- This method offers a new strategy for enhancing the performance and applicability of potentiometric sensors.
- The developed technique may significantly broaden the scope of applications for potentiometric sensing devices.