Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genes active in developing wheat endosperm.

B C Clarke1, M Hobbs, D Skylas

  • 1CSIRO Plant Industry, PO Box 1600, Canberra, ACT 2601, Australia. b.clarke@pi.csiro.au

Functional & Integrative Genomics
|January 17, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Complex problems, local solutions: Understanding systems approaches to obesity prevention in the UK.

Health & place·2026
Same author

Examining spatial variation and inequity in COVID-19 immunisation coverage in Aotearoa New Zealand: a nationwide geospatial study.

Vaccine·2026
Same author

Te Papa Hauora Future of Health Disrupt Challenge: an authentic learning and assessment opportunity.

Perspectives in public health·2025
Same author

Environmental influences on mental health: eight-year longitudinal data show a bi-directional association between residential mobility and mental health outcomes.

Health & place·2025
Same author

An observational study of intergenerational sex differences in mortality in Aotearoa New Zealand.

Social science & medicine (1982)·2025
Same author

Access to Healthy Built and Natural Environments and Physical Activity and Screen Time in New Zealand Adolescents: A Geospatial Cross-Sectional Study.

GeoHealth·2025
Same journal

Positive regulatory role of EuWRKY1 in the biosynthesis of Eucommia ulmoides rubber.

Functional & integrative genomics·2026
Same journal

Environmental enrichment modulates, but does not normalize, aging-associated immune transcriptional programs after ischemic stroke.

Functional & integrative genomics·2026
Same journal

A hypoxia-responsive migrasome-related lncRNA signature predicts prognosis and suggests the MSC-AS1/ITGA5 axis as a potential therapeutic target in head and neck squamous cell carcinoma.

Functional & integrative genomics·2026
Same journal

Genome-wide exploration of biosynthetic gene clusters and their association with virulence in the entomopathogenic fungus Beauveria.

Functional & integrative genomics·2026
Same journal

Single-cell transcriptomic analysis identifies a tumor-enriched GIMAP7⁺ CD4⁺ naïve T cell population with a unique immunoregulatory state in lung adenocarcinoma.

Functional & integrative genomics·2026
Same journal

Integrating artificial intelligence and multi-omics data for precision oncology in endometrial cancer: a narrative review.

Functional & integrative genomics·2026
See all related articles

Researchers constructed a wheat endosperm cDNA library to study early grain development. This revealed an estimated 4,500-8,000 expressed genes, including novel glutenin/gliadin variants.

Area of Science:

  • Plant Molecular Biology
  • Wheat Genetics
  • Gene Expression Analysis

Background:

  • Wheat endosperm development is crucial for grain quality and yield.
  • Understanding gene expression during early grain filling is essential for crop improvement.
  • Previous studies lacked comprehensive cDNA libraries for early wheat endosperm development.

Purpose of the Study:

  • To construct and characterize a cDNA library from early developing wheat endosperm.
  • To estimate the number of genes expressed during this critical developmental stage.
  • To identify novel genes, particularly those related to seed storage proteins.

Main Methods:

  • Characterization of wheat endosperm tissue (dry weight, cytology, mRNA timing).
  • 2D-electrophoresis to analyze protein complement.

Related Experiment Videos

  • Construction and sequencing (4,319 single-pass reads) of a non-normalized cDNA library from 8-12 days post-anthesis endosperm.
  • Bioinformatic analysis of cDNA sequences to partition into gene families and singletons.

    • Main Results:

    • A high-quality, full-length enriched cDNA library was created.
    • Mathematical modeling estimated 4,500-8,000 different genes expressed in early endosperm.
    • Approximately 27% of gene products were detected by 2D-electrophoresis.
    • A novel class of glutenin/gliadin seed storage protein genes was identified.

    Conclusions:

    • The study provides a valuable resource for wheat endosperm research.
    • Early wheat grain filling involves a complex gene expression profile.
    • Novel seed storage protein genes have implications for wheat end-use properties.