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Related Experiment Videos

Toward a fully integrated positive-pressure driven microfabricated liquid analyzer.

Paul G Vahey1, Sean A Smith, Colin D Costin

  • 1Center for Process Analytical Chemistry, Department of Chemistry, University of Washington, Seattle 98195-1700, USA.

Analytical Chemistry
|January 25, 2002
PubMed
Summary

A novel integrated analyzer offers continuous, near-real-time sampling and multiwavelength detection for flow injection analysis and capillary separations. This versatile liquid analyzer achieves high separation efficiency and sensitive detection for various analytes.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Chromatography

Background:

  • Traditional analytical methods often lack the sensitivity and real-time capabilities required for complex sample analysis.
  • Developing integrated systems for flow injection analysis and capillary separations is crucial for advancing analytical throughput and detection limits.

Purpose of the Study:

  • To describe a versatile integrated analyzer incorporating flow-programmed injection and multiwavelength detection.
  • To demonstrate the application of this analyzer in sampling, flow injection analysis, and capillary separations.
  • To showcase the utility of grating light reflection spectroscopy (GLRS) and transmission absorbance spectroscopy for refractive index and absorbance detection.

Main Methods:

  • Utilized a flow-programmed injection strategy for precise control over injection volumes (250 pL to microliters).

Related Experiment Videos

  • Employed simultaneous multiwavelength grating light reflection spectroscopy (GLRS) and transmission absorbance spectroscopy in a 150-pL detection volume.
  • Applied a siloxane coating on the GLRS grating as a sensing layer for sample-analyzed interactions.

    • Main Results:

    • Achieved a limit of detection (LOD) of 170 microM (2.6 fmol) for FD&C Red #3 using GLRS interferometric detection.
    • Demonstrated LODs of 2 mM for phosphate buffer (3 fmol) in the 150-pL detection volume.
    • Obtained a separation efficiency (N) of 16,000 for unretained food dye peaks in open tubular capillary liquid chromatography.

    Conclusions:

    • The integrated analyzer provides continuous, near-real-time sampling with high sensitivity and selectivity.
    • GLRS is a unique, path-length-independent technique for probing RI, absorbance, and optical properties.
    • The combined GLRS and chromatographic analysis offers comprehensive insight into sample properties, making it a broadly applicable liquid analyzer.