Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Automated screening for genomic imbalances using matrix-based comparative genomic hybridization.

Swen Wessendorf1, Björn Fritz, Gunnar Wrobel

  • 1Division Organisation komplexer Genome, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|January 18, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of adalimumab treatment on impairment of non-professional activities in psoriasis patients.

Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·2025
Same author

Impact of TP53 Mutation Status in Elderly AML Patients When Adding All-Trans Retinoic Acid or Valproic Acid to Decitabine.

European journal of haematology·2024
Same author

Predictors for severe persisting pain in rheumatoid arthritis are associated with pain origin and appraisal of pain.

Annals of the rheumatic diseases·2024
Same author

Epidemiology and Treatment of Patients With Primary Open Angle Glaucoma in Germany: A Health Claims Data Analysis.

Journal of glaucoma·2024
Same author

Evaluation of Nanopore Sequencing on Polar Bodies for Routine Pre-Implantation Genetic Testing for Aneuploidy.

Clinical chemistry·2024
Same author

Epidemiology and Real-World Treatment of Incident Diffuse Large B-cell Lymphoma (DLBCL): A German Claims Data Analysis.

Oncology and therapy·2024

Automated matrix comparative genomic hybridization (CGH) offers high-resolution detection of genomic imbalances in tumors. This robust method enables reliable identification of gene dosage variations, paving the way for broader clinical applications.

Area of Science:

  • Genomics
  • Molecular Biology
  • Cancer Research

Background:

  • Comparative genomic hybridization (CGH) has identified numerous tumor-specific genomic alterations.
  • Matrix-CGH offers higher resolution for detecting genomic imbalances compared to traditional CGH.
  • Previous methods were limited by sample size and automation challenges.

Purpose of the Study:

  • To develop and validate a fully automated matrix-CGH protocol.
  • To assess the feasibility of matrix-CGH from small tissue samples using universal DNA amplification.
  • To evaluate the performance of automated matrix-CGH in detecting gene dosage variations in tumor cell lines and primary tumors.

Main Methods:

  • Development of a fully automated matrix-CGH protocol using specialized instrumentation.

Related Experiment Videos

  • Design of DNA chips with 188 genomic DNA fragments (BAC/PAC clones).
  • Testing the protocol on tumor cell lines (COLO320-HSR, HL60, NGP) and a primary lymphoma, including investigation of MYC gene amplicons.

    • Main Results:

    • Automated matrix-CGH demonstrated high concordance (85%) with chromosomal CGH but with a superior dynamic range.
    • Identified homogeneous and heterogeneous amplification patterns of the MYC gene in tumor cell lines.
    • Validated alternative normalization algorithms for primary tumors lacking defined aberrations.

    Conclusions:

    • The presented automated matrix-CGH protocol provides robust and reliable detection of genomic gains and losses.
    • This automated approach facilitates widespread application in tumor and clinical genetics.
    • Matrix-CGH is a powerful tool for high-resolution analysis of genomic alterations in cancer.