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Updated: Oct 2, 2026

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors
Published on: January 20, 2023
Protective functions of intracellular heat-shock protein (HSP) 70-expression in patients with severe sepsis
S Bruemmer-Smith1, F Stüber, S Schroeder
1Department of Anesthesiology and Critical Care Medicine, Royal Brompton Hospital, Sydney Street, London SW3 6NP, UK.
Abstract:
Intracellular expression of heat-shock-protein 70 (HSP70) arose early in evolutionary development as a tool to protect cellular homeostasis. HSP70 detects proteins that are incorrectly folded or denatured. They form a complex with such proteins which can lead to correct folding, compartmentalization in organelles, or to proteolytic degradation. HSP70 also appears to protect proteins from degeneration. Intracellular HSP70-expression is induced by a wide variety of stimuli including heat, fever, hypoxia, oxygen radicals, endotoxins, cytokines, and heavy metal ions. Pre-emptive induction of HSP70-expression reduces organ dysfunction and mortality in animal models of sepsis.
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