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Related Experiment Videos

[Lactate dehydrogenase (LD)].

M Maekawa1

  • 1Department of Laboratory Medicine, Hamamatsu University School of Medicine.

Rinsho Byori. the Japanese Journal of Clinical Pathology
|January 19, 2002
PubMed
Summary
This summary is machine-generated.

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Lactate dehydrogenase (LD) activity is measured using the JSCC method. LD isozyme patterns are vital for diagnosis but can be altered by elimination rates, LD-immunoglobulin complexes, genetic variants, or tumors.

Area of Science:

  • Biochemistry and Clinical Chemistry
  • Enzyme kinetics and analysis
  • Molecular diagnostics

Context:

  • Lactate dehydrogenase (LD) is a crucial enzyme in cellular metabolism, existing as a tetramer composed of M(A) and H(B) subunits.
  • The distinct quantitative distribution of LD isozymes provides characteristic patterns.
  • These patterns are integral to laboratory diagnosis and clinical decision-making.

Purpose:

  • To elucidate the measurement of lactate dehydrogenase (LD) activities using the Japanese Society of Clinical Chemistry (JSCC) recommended method.
  • To highlight the diagnostic utility of LD isozyme patterns in clinical settings.
  • To identify factors that can modify serum LD isozyme patterns, potentially affecting diagnostic accuracy.

Summary:

  • Lactate dehydrogenase (LD) activity is quantified via the JSCC-recommended method, measuring the conversion of lactate to pyruvate.

Related Experiment Videos

  • The tetrameric structure of LD, formed by M(A) and H(B) subunits, results in distinct isozyme distributions.
  • Serum LD isozyme patterns, while diagnostically valuable, can be influenced by variable isozyme elimination rates, LD-immunoglobulin complexes, genetic variations, and tumor-associated LD production.

    • Impact:

    • Understanding LD isozyme behavior is critical for accurate interpretation of diagnostic results.
    • Recognition of modifying factors ensures reliable application of LD analysis in clinical practice.
    • This knowledge aids in differentiating true pathological findings from analytical or physiological variations.