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Related Experiment Videos

Formation of Sertoli cell-enriched tissue constructs utilizing simulated microgravity technology.

D F Cameron1, J J Hushen, S J Nazian

  • 1Department of Anatomy, University of South Florida College of Medicine, Tampa 33612, USA. dcameron@HSC.usf.edu

Annals of the New York Academy of Sciences
|January 19, 2002
PubMed
Summary
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Transplantation proceedings·2014

Sertoli cells support cell transplantation for diabetes and Parkinson's disease by protecting grafts from rejection and providing essential nutrients. This novel approach enhances cell survival and function for potential long-term therapies.

Area of Science:

  • Regenerative Medicine
  • Cell Therapy
  • Neuroscience

Background:

  • Cell transplantation shows promise for diabetes and Parkinson's disease but faces challenges like graft rejection and poor survival.
  • Sertoli cells, known for supporting germ cells, possess immunoprotective and trophic properties beneficial for transplanted cells.

Purpose of the Study:

  • To investigate the efficacy of cotransplanting Sertoli cells with islets and neurons to improve graft survival and function for diabetes and Parkinson's disease.
  • To develop novel Sertoli cell-based aggregates (SICA and SNAC) using simulated microgravity culture for enhanced cell transplantation.

Main Methods:

  • Sertoli cells were cocultured with neonatal pig islets (SICA) and immortalized neurons (SNAC) using NASA's simulated microgravity technology (HARV biochamber).

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  • Aggregates were analyzed for cell viability, structural integrity, and functional characteristics, including insulin secretion and neuronal differentiation.
  • Matrigel was added to assess its impact on aggregate structure and function.

    • Main Results:

    • Both SICA and SNAC demonstrated high cell viability (>90%) with the presence of FasL-expressing Sertoli cells.
    • Sertoli cells did not impede insulin secretion in SICA and promoted dopaminergic differentiation in SNAC.
    • Matrigel incorporation improved aggregate structure and enhanced insulin secretion.

    Conclusions:

    • Sertoli cell aggregates (SICA and SNAC), particularly when combined with Matrigel, form functional "biochambers" suitable for transplantation.
    • These Sertoli cell biochambers offer a promising strategy for long-term cell transplantation therapy in diabetes and Parkinson's disease.