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Monitoring of Systemic and Hepatic Hemodynamic Parameters in Mice
Published on: October 4, 2014
Measurement of primary haemostasis using a pressure clamp technique
V Kretschmer1, S Bade, M Weippert-Kretschmer
1Department of Transfusion Medicine and Haemostaseology, University Hospital, Marburg, Germany. kretschv@mailer.uni-marburg.de
Platelets
|January 19, 2002
Summary
A new VCP2 method offers a more reliable platelet function test than the PFA-100. It reduces test variability and expands its use to more challenging patient samples, improving bleeding risk assessment.
Area of Science:
- Hematology
- Medical Devices
Background:
- The PFA-100 is a screening test for primary hemostasis.
- It uses a capillary to provide hemodynamic resistance for aperture perfusion.
Purpose of the Study:
- To present a modified method (VCP2) for measuring platelet function.
- To replace the PFA-100's capillary with an 'electronic capillary'.
Main Methods:
- The VCP2 method clamps the pressure/flow relationship, simulating a capillary.
- It measures closure time (CT) and blood volume (BV).
Main Results:
- VCP2 showed strong correlation with PFA-100 for CT and BV (r=0.86-0.952).
- VCP2 significantly reduced test variability (CV) for CT compared to PFA-100.
- VCP2 demonstrated comparable sensitivity for von Willebrand disease (vWD) and extended applicability to thrombocytopenic samples.
Conclusions:
- The VCP2 method offers increased reliability and reduced variance in platelet function testing.
- Its adaptability through software modifications enhances its clinical utility.
- VCP2 may improve bleeding risk assessment, particularly in patients with bone marrow hypoplasia.
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