[The effect of bombesin on cyclin D1/CDK4 of immortalized human gastric epithelial cell line]
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Published on: April 20, 2017
1Department of Gastroenterology, Peking Union Medical College Hospital, Beijing 100730, China.
Objective:
To investigate the regulatory effect of bombesin on the growth of immortalized human gastric epithelial cell line GES-1, as well as on the cell cycle-regulating elements cyclin D1 and CDK4.
Methods:
GES-1 cells were treated with bombesin, alone or together with an bombesin antagonist. The growth effect was evaluated by MTT method, cyclin D1 expression by flow cytometry, CDK4 expression by Western blot plus enhanced chemoluminescence and CDK4 activity by immunoprecipitation with antibody-coated Sepharose beads.
Results:
Bombesin at 10(-7) mol/L significantly stimulated the growth of GES-1 cells, increased cyclin D1 expression which was inhibited by bombesin antagonist. Bombesin also increased CDK4 protein expression and CDK4 activity.
Conclusion:
In an immortalized human gastric epithelial cell line, bombesin promotes cell cycle progression via an increase in the expression of cyclin D1/CDK4 and CDK4 activity.
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