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[Study on the effect of COX-2's selective inhibitor on human colorectal adenoma cells proliferation]
1Department of Internal Medicine. First Hospital, West China University of Medical Sciences, Chengdu 610041, China.
Objective:
To investigate the mechanism of COX-2's selective inhibitor, NS-398, on human colorectal adenoma development.
Methods:
The human colorectal adenoma cells were isolated and cultured identified. The cell growth rates were assessed, when treated with NS-398, by using MTT method. Expression of COX-2 protein in human colorectal tumor was examined by immunohistochemistry.
Results:
23 of 30 colorectal adenomas were primary cultured successfully. NS-398 inhibited the proliferation of the cells in a dose- and time-dependent manner. COX-2 was overexpressed in colorectal adenoma(83.1%) and cancer(80.0%)compared with normal colon mucosa.
Conclusion:
The key of the several culture conditions for the survival of human colorectal adenoma cells have been improved. Such as, best of the digestive time and number of cell. The cells can be inhibited by NS-398 with dose-dependent. Overexpression of COX-2 protein occurs early during colorectal carcinogenesis which contribute to tumour formation.
Insights
The selective cyclooxygenase-2 (COX-2) inhibitor NS-398 effectively reduced human colorectal adenoma cell growth. Overexpression of COX-2 is an early event in colorectal cancer development.
Area of Science:
- Biochemistry
- Oncology
- Molecular Biology
Context:
- Colorectal adenomas are precancerous lesions.
- Cyclooxygenase-2 (COX-2) is implicated in tumor development.
- Selective COX-2 inhibitors are potential therapeutic agents.
Purpose:
- To investigate the mechanism of action of the selective COX-2 inhibitor NS-398 in human colorectal adenoma development.
- To assess the effect of NS-398 on colorectal adenoma cell proliferation.
- To examine COX-2 protein expression in colorectal tumors.
Summary:
- Human colorectal adenoma cells were successfully cultured and treated with NS-398.
- NS-398 demonstrated dose- and time-dependent inhibition of cell proliferation.
- COX-2 was found to be overexpressed in colorectal adenomas and cancers compared to normal mucosa.
Impact:
- Improved culture conditions for human colorectal adenoma cells.
- Demonstrated NS-398's inhibitory effect on colorectal adenoma cell growth.
- Established early overexpression of COX-2 in colorectal carcinogenesis, contributing to tumor formation.
